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KMID : 1024420200240010062
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Food Engineering Progress
2020 Volume.24 No. 1 p.62 ~ p.76
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A Study of the Diversity and Profile for Enzyme Production of Aerobically Cultured Halophilic Microorganisms from the Various Solar Salterns
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Lee Yong-Jik
Shin Kee-Sun
Lee Sang-Jae
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Abstract
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This research confirmed the diversity and characterization of halophilic microorganisms isolated from the various solar salterns, collected on the inside and outside of the country. To isolate strains, the marine agar medium was basically used and cultivated at 37oC for several days aerobically. After single colony isolation, a totally of 230 pure colonies were isolated and phylogenetic analysis was carried out based on the result of 16S rDNA sequencing, indicating that isolated strains were divided into 4 phyla, 12 families, 27 genera and 64 species. Firmicutes phylum, the main phyletic group, comprised 89.6% with 3 families, 17 genera and 52 species of Bacillaceae, Staphylococcaceae and Carnobacteriaceae. To confirm whether isolated strain can produce industrially useful enzyme or not, amylase, lipase, and protease enzyme assays were performed individually, showing that 177 strains possessed at least one enzyme activity. Especially 17 strains showed all enzyme activity tested. This result indicated that isolated strains have shown the possibility of the industrial application. Therefore, this study has contributed to securing domestic genetic resources and the expansion of scientific knowledge of the halophilic microorganisms community in solar salterns.
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KEYWORD
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Salt, Halophilic, Diversity, Extracellular enzyme, Bacteria
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